OL- 109.5 - Laboratory Procedures for Microbiology
(Lab for Microbiology)

The EPA has indicated that there is no safe level for Coliform Bacteria. Therefore:

 

The MCL for COLIFORM BACTERIA IS: ZERO (0) under the Presence-Absence Rule.

 

  • Multiple Tube Fermentation (MTF) Technique utilizing 5 tubes of 20 ml. Sample each or 10 tubes of 10 ml. sample each.

 

  • Presence-Absence (P-A) Coliform Test utilizing a single 100 ml. sample volume

 

  • Membrane Filter (MF) Technique utilizing a 100 ml. filter volume.

 

  • Minimal Media ONPG-MUG or Autoanalysis Colilert System).